FIG 2 .
Deletion of sskA and genes encoding MAPKs results in reduced asexual development at 25°C. (A) Colonies of a wild-type (WT) control strain (ΔligD pyrGt) and ΔsskA, ΔsakA, ΔmpkA, and ΔmpkB strains grown on ANM plus supplements plus GABA or ammonium (NH4) at 25°C for 14 days. (B) Quantification of the conidial density (conidia × 105 per mm2) of strains grown on either GABA or ammonium as the sole nitrogen source after 10 days at 25°C. Statistical significance using an unpaired t test and 95% confidence interval is indicated by asterisks as follows: **, P < 0.005; ***, P < 0.001. (C) Conidial preparations of the wild type and the ΔmpkB mutant grown on ammonium as a nitrogen source after 10 days at 25°C. The ΔmpkB mutant inappropriately produces yeast cells (white arrows) in addition to conidia (white arrowheads). Images with epifluorescence to observe calcofluor white-stained fungal cell walls are shown. Bars, 10 µm.