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Mouse neutralization assay using serial dilutions of polyclonal equine anti-A1 antitoxina
| Antibody dilution | No. of mice that survived up to 96 h/total no. of mice injected with the following toxin: |
|
|---|---|---|
| BoNT/FA | BoNT/A1 | |
| 100 | 4/4 | |
| 200 | 4/4 | |
| 400 | 0/4 | |
| 800 | 0/4 | |
| 1,000 | 4/4 | |
| 1,600 | 0/4 | |
| 2,000 | 4/4 | |
| 4,000 | 4/4 | |
| 8,000 | 0/4 | |
| 16,000 | 0/4 | |
The mice were injected with BoNT/FA and BoNT/A1 toxin at 500 mLD50 per mouse. Note that all animal deaths occurred within the first 24 h (i.e., no delayed deaths).
