TABLE 2 .
Designation of group-specific OTU significantly different between microbiotas of FlaC-treated and mock-treated chickens
| Specificity and OTUa | Class or order | Family | Genus (cluster) | Most closely related sequence (% nucleotide identity) |
|---|---|---|---|---|
| Mock-treated group | ||||
| OTU 63 | Erysipelotrichales | Erysipelotrichaceae | Erysipelatoclostridium | Clostridium innocuum (93) |
| OTU 73 | Lactobacillales | Lactobacillaceae | Lactobacillus | Lactobacillus ingluviei (98) |
| OTU 87 | Clostridiales | Clostridiaceae | Clostridium (cluster IV) | Clostridium sp. strain YIT12069 (100) |
| OTU 134 | Clostridiales | Oscillospiraceae | Oscillibacter | ND |
| FlaC-treated group | ||||
| OTU 50 | Erysipelotrichales | Erysipelotrichaceae | Clostridium (cluster XVIII) | ND |
| OTU 52 | Clostridiales | Lachnospiraceae | Blautia | Blautia faecis (98) |
| OTU 121 | Clostridiales | Lachnospiraceae | Blautia | Blautia hydrogenotrophica (97) |
Designation of group-specific OTU at the genus or species level was verified by nucleotide BLAST search of 16S rRNA gene amplicon sequences using the NCBI nr database or SINA alignment with sequences listed in the Silva, Living Tree Project (LTP), or Ribosomal Database Project (RDP) database (http://www.arb-silva.de). Noted identity scores (percentages) reflect similarities of OTU-specific consensus nucleotide sequences with the identified closest bacterial relatives. ND, not determined due to low similarity (<90%) of database hits.