TABLE 3 .
Oligonucleotides used for gene amplification and cloning
| Gene | Primers | Sequence (5′–3′)a | Tm (°C) | Reference |
|---|---|---|---|---|
| flaC (Cj0720c) | CjflaC_F_BamHI | AAAGGATCCCAAAGTGGCTTAATGATGACG | 55 | This study |
| CjflaC_R_BamHI | AAAGGATCCCGCTAGAGCTTGGACTTGAT | 55 | This study | |
| CjflaC_F3 | AAAGGATCCATCTCTGATGCAACTATGATG | 53 | This study | |
| CjflaC_R2 | AAACTCGAGTTGTAATAAATTAGCAATTTTGC | 51 | This study | |
| rdxA (Cjj81176_1083) | CjrdxA_ClaI_R3 | AAAATCGATGTTGATTGTAACATAGGGTTG | 51 | This study |
| CjrdxA_SpeI_F3 | AAAACTAGTCAAGTGCGAGTCATAATATC | 51 | This study | |
| CjrdxA_XbaI_F1 | AAATCTAGAGTGATTTTGTCGTAGATGAAG | 53 | This study | |
| CjrdxA_XbaI_R1 | AAATCTAGATATAAATTTCCAAGGTTCCA | 51 | This study | |
| SalTy LT2 fliC | SalLT2FliCBamHI(F) | CGGGATCCATGGCACAAGTCATTAATAC | 49 | This study |
| SalT2FliCEcoRI(R) | CGGAATTCCGCAGTAAAGAGAGGACG | 51 | This study | |
| chTLR5 | chTLR5_F1 | AAAGGTACCGAGTCCGGATCCATGATGTTACAATCAA CGGCTAATAATTG |
65 | This study |
| chTLR5_R1 | AAAAGCGGCCGCCGTGTGAGACTGTCGCTATAGTTTG | 69 | This study | |
| CAT cassette | pCAT1 | AACAGCTATGACCATGATTAC | 57 | 81 |
| pCAT2_BamHI | AGAGGATCCGATATCGCATGCCTGCAGAG | 57 | 81 | |
| Gm cassette (aac) | Gm1_ClaI | AAAATCGATCGGGTGACTAACTAGGAGGAATAA | 65 | This study |
| Gm2_SpeI | AGAACTAGTCCGTGTCATTATTCCCTCCAGGTA | 67 | This study |
a
Restriction sites in oligonucleotides are underlined. Tm, melting temperature.