TABLE 2 .
RT-qPCR primers
| Gene name | Assembly 19/21 identifier | Primer sequence |
Temp (°C) |
||
|---|---|---|---|---|---|
| Forward primer | Reverse primer | Annealing | Reading | ||
| ACT1 | orf19.5007 | GTTGGTGATGAAGCCCAATC | CCCAGTTGGAAACAATACCG | 62 | 72 |
| CDC19 | orf19.3575 | TTGTCCACCAGTGGTCTTTC | AGCAGCTCTTTCGTTTCTGG | 60 | 70 |
| CTN1 | orf19.4551 | GGATTTGGTCCAGTTTCTGC | TGACGATGTTTGGAACTAGCAC | 60 | 70 |
| CTN3 | orf19.2809 | CTTTCCAGTGCCATGTCTTC | TGTTGCGGTAAGTGGCTATC | 61 | 75 |
| ICL1 | orf19.6844 | CAGCATTGGCTGTTGATGAC | TGGACAGTTTGACCGTAAGC | 60 | 70 |
| LSC2 | orf19.1860 | AGCTGAAGCCGGTAAATACG | GCCAAACCAGCACCATTAAC | 60 | 73 |
| MLS1 | orf19.4833 | CAGCTTTGGTTCCAGTTGTG | GTTCCTTTGGTGGCAATGAG | 60 | 72 |
| PMA1 | orf19.5383 | CCGAAGCCTTTGACAACTTC | CTCTTTGCATGGACACAAGG | 60 | 73 |
| POT1 | orf19.7520 | TGGCTGAAGCCAAAGGTTAC | GGACCAACACCCATGATTTC | 61 | 75 |
| POX1-3 | orf19.1652 | GAAGGCTCATCACTACCTGTTG | GCAATGGTGAGCAAGTATGG | 60 | 72 |