FIG 1.
Observation of prospore membranes in wild-type (WT) and spo73∆ and spo71∆ mutant cells. (A) Overview of the stages of prospore membrane formation. SPB, spindle pole body. (B) Wild-type and vps13∆, spo73∆, and spo71∆ mutant cells in the BY4743 background expressing GFP-SPO2051-91 were sporulated for 24 to 36 h and subjected to fluorescence microscopy. (C) AN120 (wild-type), TC545 (spo73∆ mutant), and TC581 (spo71∆ mutant) cells expressing GFP-SPO2051-91 were sporulated for 6.5 h and subjected to time-lapse microscopic analysis. The time after the start of observation is shown in minutes. Scale bars, 5 μm.