Skip to main content
. Author manuscript; available in PMC: 2016 May 11.
Published in final edited form as: Science. 2015 Oct 22;350(6259):450–454. doi: 10.1126/science.aac7444

Fig. 4. PUB4 is part of a chloroplast quality-control pathway.

Fig. 4

(A) Quantification of degrading chloroplasts using scanning electron microscopy micrographs (19) of cotyledon mesophyll cells of 5-day-old seedlings grown in constant light (n ≥ 54 cells). (B to E) TEM images of chloroplasts undergoing various levels of degradation in fc2-1 seedlings. Scale bars, 1 μm. (F) Quantification of chloroplasts in cotyledon palisade mesophyll cells (n ≥ 6 seedlings) and (G) chlorophyll levels (n = 3 replicates) of 6-day-old seedlings grown in constant light. (H) Senescing rosette leaves of 5-week-old plants grown in constant light. (I) Phenotypes and fresh weight (FW) of seedlings (n = 7 groups of 10) grown for 1 week in low light (30 μmol · m−2 · s−1 at 22°C) and then transferred to excess light (275 μmol · m−2 · s−1 at 15°C) for 1 week. All values are means T SEM. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, two-tailed Student’s t test.