Figure 4. Distribution of protein coexpression clusters.

(A) Relative frequency of tumor cells representing each cluster within the complete data set (638,577 IDC cells from 26 human breast cancers). (B) Intertumoral distribution of cells representing each protein coexpression cluster. Each row in the table represents one tumor sample, and each column represents one protein coexpression cluster. Tumors were sorted by the extent of intratumoral heterogeneity, with the most homogenous tumor on top (99% of tumor cells expressing cluster C6) and the most heterogenous tumor at the bottom (coexistence of cancer cells representing 5 different protein coexpression clusters, with 3 clusters [C1, C2, and C4] contributing about one-third of the tumor cells each). The number in each cell indicates the percentage of neoplastic cells expressing a particular protein coexpression cluster. The percentage of cells expressing the most abundant cluster is shown in the area graph to the right and is 50% or greater (dashed line) in most tumors. (C) Intratumoral distribution of cells representing each protein coexpression cluster. Pie charts show the distribution of protein expression clusters in each field of view. Shown are 3 representative patterns. See Supplemental Figure 5 for a complete view of all breast cancer samples. (D) FDG tumor uptake in 26 patients with invasive ductal breast cancer. FDG uptake was measured by PET and quantified as standardized uptake values (SUVmax).