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. Author manuscript; available in PMC: 2016 May 11.
Published in final edited form as: Lab Invest. 2015 May 11;95(7):817–832. doi: 10.1038/labinvest.2015.57

Figure 1.

Figure 1

miR-129-5p, epithelial–mesenchymal transition-related genes, SIP1, SOX4, and TGF-β1 expression in the mesothelial cells isolated from effluent of patients with peritoneal dialysis (PD). (a) MicroRNA (miRNA) expression profiling in the mesothelial cells isolated from effluent of patients with PD, as detected by miRNA array analyses, a decreased expression of miR-129-5p was seen (asterisks). (b, B1) Northern blot analysis showed that miR-129-5p expression decreased in the PD patients (PD<6 months) as compared with that of the control group (PD<6months). There was no change in U6snRNA expression, which served as an internal control. (B2) The bar graph represents the summary of the messenger RNA (mRNA) expression of miR-129-5p from northern blot analysis. (c) The bar graph represents the summary of the mRNA expression of E-cadherin, claudin-1, vimentin (C1), fibronectin, SIP1, and SOX4 (C2) in the cells by real-time PCR. (d) Expression profiling of their protein levels as assessed by western blot analysis. (e, E1–E3) The bar graphs represent the density of relative bands depicted by western blot analyses. (f) TGF-β1 expression in the effluent of PD patients by an enzyme-linked immunosorbent assay. Values are the mean ± s.e.m., n = 4, *P<0.01 vs control (PD<6 months).