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. Author manuscript; available in PMC: 2016 May 11.
Published in final edited form as: Lab Invest. 2015 May 11;95(7):817–832. doi: 10.1038/labinvest.2015.57

Figure 2.

Figure 2

Expression of epithelial–mesenchymal transition-related genes, SOX4, and SIP1 in human peritoneal mesothelial cell lines (HPMCs) induced by TGF-β1. (a) The bar graph represents the summary of the results by real-time PCR. (A1) An overt decrease in messenger RNA expression of miRNA-129-5p was observed in HPMCs, a human peritoneal mesothelial cell line, following treatment with TGF-β1. Real-time PCR showed that E-cadherin (A2) and Claudin-1(A3) expressions were significantly decreased in a time-dependent manner in HPMCs treated with TGF-β1, whereas expression of vimentin (A4) and fibronectin (A5), SIP1 (A6) and SOX4 (A7) were upregulated. (b) Western blot assays showed the expression of E-cadherin, claudin, vimentin, fibronectin (FN), SIP1, and SOX4 proteins in HPMCs induced with TGF-β1. (c, C1–C6) The bar graphs representing the western blot relative band densities from Figure 7b. Values are the mean ± s.e.m., n = 3, *P<0.01 vs control.