Skip to main content
. Author manuscript; available in PMC: 2016 May 11.
Published in final edited form as: Lab Invest. 2015 May 11;95(7):817–832. doi: 10.1038/labinvest.2015.57

Figure 4.

Figure 4

SIP1-siRNA reverses the altered expression of epithelial–mesenchymal transition-related genes in human peritoneal mesothelial cell lines (HPMCs) induced by TGF-β1. (a) By real-time PCR, a decreased messenger RNA (mRNA) expression of E-cadherin was seen in HPMCs subjected to TGF-β1 treatment (5 ng/ml) for 48 h, whereas it was reversed by transfection with pre-miR-129-5p or SIP1-siRNA. Inhibition of SIP1 expression further enhanced the reversal effect of pre-miR-129-5p in TGF-β1-reduced E-cadherin mRNA expression (A1). In addition, compared with E-cadherin expression, an opposite effect was observed for vimentin and fibronectin (FN) mRNA expression (A2, A3). (b) Western blot analysis showed similar results that SIP1-siRNA reversed the altered expression of E-cadherin, vimentin, and FN in HPMCs subjected to TGF-β1 treatment. The bar graphs represent the densitometric measurements of the relative band density observed by western blot analyses (c, C1–C3). (d, D1) Western blot analysis revealed that overexpression of pre-miR-129-5p partially blocked the altered protein expression of E-cadherin and vimentin in HPMCs treated with TGF-β1 or TGF-β1 +SIP1. (D2) The bar graphs represent the densitometry measurements of relative band density of autoradiograms of D1. Values are the mean ± s.e.m., n = 3, *P<0.01 vs control, P<0.01 vs TGF-β1, P<0.01 vs $P<0.01 vs TGF-β1+pre-miR-129-5p.