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. Author manuscript; available in PMC: 2016 May 11.
Published in final edited form as: Lab Invest. 2015 May 11;95(7):817–832. doi: 10.1038/labinvest.2015.57

Figure 6.

Figure 6

Effect of miR-129-5p on the expression of SIP1 and SOX4 in human peritoneal mesothelial cell lines (HPMCs) treated with TGF-β1. (a and b) By real-time PCR, a significant increase in the SIP1 and SOX4 messenger RNA expression in HPMCs subjected to TGF-β1 treatment was seen, whereas it was not significantly different between the TGF-β1-treated group or group co-treated with TGF-β1+miR-129-5p. (c) Immunofluorescence microscopy revealed an increased SIP1 and SOX4 expression in the nuclei of HPMCs treated with TGF-β1, which was normalized with transfection with pre-miR-129-5p. Magnification, × 400. (d) Similar results were observed by western blot assays. (D1, D2) The bar graphs represent the expression of SIP1 and SOX4 protein band densities relative to β-actin. Values are the mean ± s.e.m., n = 3, *P<0.01 vs control, #P<0.01 vs TGF-β1.