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. Author manuscript; available in PMC: 2016 May 11.
Published in final edited form as: Lab Invest. 2015 May 11;95(7):817–832. doi: 10.1038/labinvest.2015.57

Figure 7.

Figure 7

miR-129-5p is predicted to directly target SIP1/SOX4 genes and modulates the promoter activity of both E-cadherin and vimentin in HPMCs. (a) Location of the predicted miR-129-5p target site in SIP1 and SOX4 3′UTR, as assessed by biological analysis (www. microrna.org). (b) A decreased luciferase activity of SIP1-wt in HPMCs treated with pre-miRNA-129-5p while the decreased activity was partially restored with co-treatment of TGF-β. There was no effect in cells transfected with miR-neg control. (c) Similar results were seen for SOX4 activity. Luciferase activities were normalized to β-gal activities. Results were obtained from three independent experiments. Data are shown as mean ± s.e.m. *P<0.01 vs control, #P<0.01 vs pre-miR-129-5p. (d and e) The bar graphs represent luciferase activity in HPMC following various treatments. Opposite effects were observed for the E-cadherin and vimentin promoters. Luciferase activity was normalized with β-galactosidase activity, and it is depicted as mean value ± s.e.m. from triplicate measurements. Data are shown as mean ± s.e.m. *P<0.01 vs control, #P<0.01 vs TGF-β1, &P<0.05 vs SIP1.