Table 2. Characterization of RD-MolPack-SIN-GFP producer cells.
| RD3-MolPack packaging cells | Picked/screened colonies | RD3-MolPack producer cells | Viability (%)a | Titer (TU/ml) | Titer (TU/cell/day) | p24Gag (ng/ml) | Infectivity (TU/ng) |
|---|---|---|---|---|---|---|---|
| PK-7-RD314 | 60b/45c | RD3-MolPack1e | 93 | 2.8 × 104 ± 9.2 × 103 | 0.0056 ± 0.0027 | 13.0 ± 6.20 | 3.8 × 103 ± 2.6 × 103 |
| PK-7-RD28 | 58/15d | RD3-MolPack24f | 90 | 3.7 × 105 ± 1.5 × 105 | 0.074 ± 0.039 | 38.3 ± 11.1 | 8.5 × 103 ± 1.5 × 103 |
| PK-7-RD28 | 11/7d | RD3-MolPack28g | 80 | 2.2 × 105 ± 5.4 × 104 | 0.043 ± 0.011 | 17.5 ± 3.40 | 1.2 × 104 ± 2.1 × 103 |
| RD2-MolPack packaging cells | Picked/screened colonies No. | RD2-MolPack producer cells | Viability (%)a | Titer (TU/ml) | Titer (TU/cell/day) | p24Gag (ng/ml) | Infectivity (TU/ng) |
| PK-7-Tat7-RD19 | 68/23d | RD2-MolPack64f | 91 | 7.0 × 104 ± 7.5 × 103 | 0.056 ± 0.009 | 40.4 ± 4.4 | 1.8 × 103 ± 1.4 × 102 |
a
Viability of the cells at the time of supernatant harvest (24 hours after cell seeding).
b
Number of picked-up colonies after zeocin selection.
c
Number of screened clones after limiting dilution cloning.
d
Number of picked/screened colonies after zeocin selection.
e
Mean ± standard error of the mean (SEM) of n = 3.
f
Mean ± SEM of n = 6.
g
Mean ± SEM of n = 3.
TU, transducing unit.