Table 3. RD3-MolPack24 LV potency after cell culture optimization and concentration.
| Condition | Titer (TU/ml) | Titer (TU/cell/day) | p24Gag (ng/ml) | Infectivity (TU/ng) |
|---|---|---|---|---|
| Raw supernatanta | 3.7 × 105 ± 1.5 × 105 | 0.074 ± 0.039 | 42.2 ± 12.7 | 8.1 × 103 ± 1.8 × 103 |
| Optimized culture conditionsb | 1.8 × 106 ± 3.5 × 105 | 0.38 ± 0.075 | 256 ± 23.2 | 6.2 × 103 ± 1.2 × 103 |
| Low-speed concentrationc | 2.4 × 108 ± 1.9 × 108 | n.a. | 2.7 × 104 ± 2.1 × 104 | 7.8 × 103 ± 3.0 × 103 |
| High-speed concentrationd | 8.6 × 107 ± 3.8 × 107 | n.a. | 1.8 × 104 ± 0.5 × 104 | 4.8 × 103 ± 2.2 × 103 |
a
As in Table 2.
b
After optimization of the culture conditions as described in Materials and Methods. Mean ± standard error of the mean (SEM) of n = 6.
c
After 100 × concentration at low-speed centrifugation. Mean ± SEM of n = 3. Yield of concentration step: total TU = 79.3 ± 24.7%; total p24Gag = 59.4 ± 7.6%.
d
After 100 × concentration at high-speed centrifugation. Mean ± SEM of n = 3. Yield of concentration step: total TU = 53.0 ± 17.3%; total p24Gag = 62.7 ± 4.8%.
n.a., not applicable.