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. 2016 May 1;30(9):1101–1115. doi: 10.1101/gad.275685.115

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© 2016 Novo et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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Figure 2. — The timing of chromatin remodeling upon ESC differentiation is consistent with a role for Nanog in orchestrating these nuclear organization events. (A) Western blot of Nanog^–/–, wild-type (WT), and Nanog-overexpressing ESCs over 5 d of differentiation with NANOG and OCT4 antibodies. (B) Chromocenter organization revealed by immunofluorescent analysis of H3K9me3 during ESC differentiation. (Dashed line) Nuclear periphery. Bar, 2 µm. (C) Box and whisker plots show the number (left) and size (right) of H3K9me3 foci per nucleus. Data were compared using a one-way ANOVA followed by Bonferroni's multiple comparison test. (n.s.) P > 0.1; (*) P < 0.01. n > 50 per time point.