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. 2016 May 6;95(18):e3559. doi: 10.1097/MD.0000000000003559

FIGURE 1.

FIGURE 1

Amitriptyline (AM)-facilitated neuronal growth of DRG neurons. A, In postnatal 1-day-old rat, dorsal root ganglion (DRG) was cultured in 6-well plate and treated with different concentrations of AM (0, 0.5, 10, 100 μM) for 12 hours. Immunostaining of Tuj-1 antibody identified the neurites of DRG neurons in vitro. As compared with the treatment without AM (0 μM), there was moderate neurite outgrowth by 0.5 μM AM and significant neurite outgrowth by 10 μM AM. However, 100 μM induced toxicity in DRG neurons. B, AM-induced neuronal growth of DRG neurons was plotted in concentration-dependent manner. Four concentrations used in A were indicated (arrows). It showed AM aided neuronal growth of DRG neurons in concentration-dependent manner till 10 μM. After that, higher concentrations of AM had toxic effect on DRG neurons.