FIGURE 3.

Amitriptyline (AM) regrew neurite and growth cone in lidocaine-injured DRG neurons. DRG culture was treated with 5 mM lidocaine for 1 hour. After medium replenishment, DRG was maintained for another 24 hours with the addition of AM (0, 0.5, or 10 μM). A, Immunohistochemical assay with Tuj-1 staining was conducted on DRG culture without AM treatment. Collapsed growth cones were identified (arrows). B, In lidocaine-injured DRG culture, then treated with 0.5 μM AM, regrown neurites were identified (arrows). C, In lidocaine-injured DRG culture, then treated with 10 μM AM, regrown growth cones were identified (arrows). D, AM-induced neuronal growth in DRG neurons after lidocaine-induced injury was quantified (^∗P < 0.05, vs 0 μM AM). DRG = dorsal root ganglion.