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. 2016 May 6;95(18):e3559. doi: 10.1097/MD.0000000000003559

FIGURE 4.

FIGURE 4

Down-regulation of TrkA blocked amitriptyline (AM)-mediated regrowth of lidocaine-injured DRG neurons. DRG culture was transfected with 100 nM TrkA-specific siRNA (TrkA-siRNA), TrkB-specific siRNA (TrkB-siRNA), or a control siRNA (C-siRNA) for 24 hours. qRT-PCR was conducted to compare the posttransfection mRNA expressions of TrkA (A) and TrkB (B) (∗P < 0.05, ΔP > 0.05). In DRG culture transfected with C-siRNA (C), TrkA-siRNA (D), or TrkB-siRNA (E), 5 mM lidocaine was added for 1 hour, followed by 24 hours incubation of 10 μM AM. Immunohistochemical assay with Tuj-1 staining was then conducted and growth cones were identified (arrows). F, The relative neurite growth in DRG neurons pretreated with siRNAs were compared (∗P < 0.05, ΔP > 0.05). DRG = dorsal root ganglion, qRT-PCR = quantitative real-time polymerase chain reaction, TrkA = tyrosine kinases receptor A, TrkB = tyrosine kinases receptor B.