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. 2015 Sep 7;7(2):165–180. doi: 10.1002/jcsm.12057

Figure 1.

Figure 1

An increase in muscle mass was found in muscle‐specific RING‐finger (MuRF)2 and MuRF3 double knockout (DKO) mice. (A) Immunoblotting of proteins from soleus (Sol) and extensor digitorum longus (EDL) from control and DKO mice using anti‐MuRF2 and anti‐MuRF3 antibody, as indicated, confirmed absence of MuRF2 and/or MuRF3 proteins in the respective single and double knockout mice. Actin served as loading control. (B) Quantification of body weight of male 7‐ to 22‐week‐old control (n = 30) and DKO (n = 26) mice. ***P < 0.001. (C) Body composition of 5‐month‐old male control (n = 8) and DKO (n = 4) mice was analysed by nuclear magnetic resonance spectroscopy. The amount of body fat, free water, and total muscle weight is shown and expressed in percent of body weight. *P < 0.05. (D) Mass of Sol and EDL normalized to tibia length of control (n = 30) and DKO (n = 26) mice. Data are shown as mean ± SEM.