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mRNA expression of neuron-specific enolase (NSE) and neurofilament protein (NF-H) in tetramethylpyrazine-inducing neuron-like cells before differentiation (blank), and up to 6 h of differentiation. Top panel shows electrophoresis of the polymerase chain reaction-amplified products expressing NSE and NF-H genes. Quantitative mRNA expression levels of genes normalized by GAPDH are presented in the lower panel.
