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The primer sequences used for RT-polymerase chain reaction analysis in this study.
| Genes | Primer sequences | Sizes (bp) | Temperature (°C) |
|---|---|---|---|
| NSE | F: GGCACTCTACCAGGACTTTG R: GCGATGACTCACCATAACCC |
398 | 61.7 |
| NF-H | F: TGAACACAGACGCTATGCGCTCAG R: CACCTTTATGTGAGTGGACACAGAG |
286 | 61.2 |
| GAPDH | F: AGAAGGCTGGGGCTCATTTG R: AGGGGCCATCCACAGTCTTC |
258 | 64.0 |
NSE, neuron-specific enolase; NF-H, neurofilament protein; F, forward; R, reverse.
