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. Author manuscript; available in PMC: 2016 May 12.
Published in final edited form as: Oncogene. 2012 Apr 9;32(7):861–871. doi: 10.1038/onc.2012.105

Figure 5.

Figure 5

eIF4E suppression reduces both pulmonary colonization and metastasis growth. (a) eIF4E suppression decreases the number of pulmonary metastatic lesions following injection of sh4E.389- and shFLuc.1309-infected TM15 cells into tail vein of nude mice. The number of metastatic lesions was determined as described in the Materials and methods. *P<0.005. (b) eIF4E suppression reduces pulmonary metastatic burden. Inducible sh4E.389- and shFLuc.1309-infected TM15 cells were injected into tail vein of DOX-treated nude mice and metastatic burden determined as described in the Materials and methods. *P<0.001. (c–e) DOX-inducible sh4E.389- and shFLuc.1309-infected TM15 cells were injected into tail vein of DOX-treated nude mice and metastatic number (c), burden (d) and average size (e) determined at 8 and 12 days. *P<0.05; **P<0.001. (f) Representative hematoxylin and eosin (H&E)-stained lung sections from DOX-treated nude mice injected with inducible sh4E.389- and shFLuc.1309-infected TM15 cells. Arrows highlight a metastatic lesion. Bar, 50 μm.