Skip to main content
. Author manuscript; available in PMC: 2016 May 12.
Published in final edited form as: Bio Protoc. 2015 Sep 5;5(17):e1586. doi: 10.21769/bioprotoc.1586

Figure 1. EPR detection of S. aureus ROS production.

Figure 1

A. Following inoculation of S. aureus to an initial OD600 of 0.06, cultures were incubated aerobically (flask: volume ratio = 10:1, 250 rpm). Culture samples for EPR analysis were withdrawn at 3 h, 24 h and 72 h of bacterial growth. B. S. aureus culture samples (72 h) were treated with either 400 units of SOD, 20 mM DMTU, or vehicle (wild-type, WT) before addition of the spin probe, CMH. The basal extent of CMH autoxidation in KDD buffer (blue line) was determined as control.