Fig 3. Inhibition of TLR4 reverses the LPS-induced phase-shift.

Peritoneal macrophages were isolated from mPer2^luciferase transgenic mice and subjected to synchronization via dexamethasone shock. 20ng/ml LPS with or without LPS-RS (5μg/ml) was added to the luminescence buffer at Time 0. (A) Bioluminescence were recorded every 2h continuing for 68 hours. (B) Oscillation curves were analyzed by cosinor and acrophase compared by one-way Anova with Bonferroni post hoc correction (mean ± SEM, n = 8, *p<0.05, versus Control, ns p>0.05, versus LPS-RS).