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. 2016 May 11;11(5):e0155075. doi: 10.1371/journal.pone.0155075

Fig 8. Circadian clock disruption alters the migration and adhesion of macrophages.

Fig 8

(A) The effect of circadian disruption on macrophage migration was evaluated using the Oris Cell Migration Assay. Peritoneal macrophages were isolated from WT and Per-TKO mice and subjected to the indicated treatment (LPS 100ng/ml) for 24h, cells stained with calcein AM for 30 min and the fluorescence signal in the migration zone quantified. (mean ± SEM n = 4–5, two-way ANOVA with Bonferroni post hoc correction, *p<0.05 versus control, # p<0.05 versus WT). (B) Fluorescently labeled peritoneal macrophages were incubated with activated adherent human aortic endothelial cells for 15 minutes at 37°C and the degree of cell adhesion assay was quantified (mean ± SEM n = 6, two-way ANOVA with Bonferroni post hoc correction, *p<0.05 versus control, # p<0.05 versus WT).