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. Author manuscript; available in PMC: 2016 Nov 18.
Published in final edited form as: Neuron. 2015 Nov 18;88(4):762–773. doi: 10.1016/j.neuron.2015.10.039

Figure 2. Elevating endogenous ChI activity with DREADD hM3D(q) enhances dSPN LTD induction.

Figure 2

(A) ChAT Cre-dependent expression of DREADD hM3D(q) in ChIs (mCitrine reporter) in D1tdTomato BAC mouse. (B) DREADD cognate ligand CNO (10 μM) increased ChI spontaneous discharge rate recorded in cell-attached patches. (C) Box plot summary of the increase of discharge rate of ChIs. Box plot boxes indicate upper and lower quartiles; whiskers specify upper and lower 90%. **p < 0.01. (D) and (E) Enhancing local cholinergic signaling promoted the induction of LTD at neighboring dSPN glutamatergic synapses. Plot of the average EPSP amplitudes as a function of time. Error bars indicate SEM. The LTD was disrupted by (D) the M4R antagonist MT3 (100 nM) or (E) the CB1R antagonist AM251 (2 μM). Solid line (average) and gray shadow (± SEM) are control LTD from the panel (D) for reference.