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The short-term exposure of MCF-12A cells to a high dose of ethanol upregulates the transcriptional expression of the metallothionein (MT) gene family. Cultures were maintained for 7 days in the presence of 25 mM ethanol, 2.5 mM acetaldehyde (fresh daily), or no addition. Above: RNA was isolated from triplicate cultures of control cells (left side) and ethanol treated cells (right side) and submitted to RT/PCR. Below: Densitometry for triplicate samples *p<0.05.
