Table II.
The short-term exposure of MCF-12A cells to high dose ethanol or acetaldehyde changes the transcriptional expression balance among members of the serpin, ankyrin, and alcohol metabolism gene families.a
| Ethanol (Eth) | Acetaldehyde (Act) | ||||
|---|---|---|---|---|---|
|
|
|
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| Gene name | Gene symbol | Eth/cont ratio | C value | Act/cont ratio | C value |
| Alcohol metabolism | |||||
| Aldehyde Dhd1A3 | ALDH1A3 | 0.80 | 1191 | 1.91 | 376 |
| Aldehyde Dhd3B2 | ALDH3B2 | 0.19 | 477 | 0.69 | 226 |
| Aldehyde Ox 1 | AOX1 | 1.91 | 138 | 1.86 | 213 |
| Serpins | |||||
| Serpin A1 | A1 | 4.41 | 314 | 0.98 | 504 |
| Serpin B2 | B2 (PAI 2) | 0.14 | 2625 | 2.04 | 495 |
| Serpin B3 | B3 | 0.10 | 2267 | 1.14 | 754 |
| Serpin B7 | B7 | 0.11 | 886 | 0.76 | 41 |
| Ankyrins | |||||
| Ankyrin 36B | ANKRD 36Bb | 2.07 | 104 | 1.60 | 152 |
| Ankyrin 22 | ANKRD 22 | 0.17 | 1027 | 0.72 | 67 |
| Epithelial/mesenchymal transition | |||||
| Cadherin 11 | CDH11 | 3.25 | 153 | 0.97 | 42 |
| Interleuk 31 rec A | IL3IRA | 2.72 | 125 | 1.49 | 62 |
| Connect tiss gf | CTGF | 2.67 | 284 | 0.91 | 748 |
| Integrin sub b6 | ITGB6 | 2.53 | 236 | 1.06 | 118 |
| Laminin β3 | LAMB3 | 2.46 | 1103 | 1.50 | 1822 |
| Matrix metl pep 2 | MMP2 | 2.34 | 210 | 1.09 | 495 |
| Thrombospondin 1 | THBS1 | 2.15 | 2410 | 1.36 | 1045 |
| Met assoc lung ad | MALAT1b | 1.86 | 388 | 1.67 | 2942 |
| Interleukin 6 | IL6 | 2.12 | 81 | 1.42 | 119 |
| Trans GFβ2 | TGFB2 | 2.09 | 413 | 1.36 | 856 |
| Cadherin 4 | CDH4 | 2.00 | 213 | 0.88 | 145 |
| Keratin 17 | KRT 17b | 0.51 | 10650 | 0.55 | 5277 |
| Keratin 78 | KRT 78 | 0.27 | 314 | 1.14 | 101 |
| Keratin 23 | KRT 23 | 0.24 | 228 | 0.76 | 127 |
| Keratin 80 | KRT 80 | 0.22 | 727 | 0.98 | 100 |
| Keratin 16 | KRT 16b | 0.28 | 2923 | 0.49 | 1850 |
| Keratin 4 | KRT 4 | 0.11 | 1197 | 0.71 | 176 |
Cultures were maintained for 7 days in the presence of 25 mM ethanol, 2.5 mM acetaldehyde (fresh daily), or no addition (cont). RNA was subjected to DNA microarray analysis by Affymetrix human Gene 1.0 ST.
A few genes have multiple probe sets in this system, so results were averaged. Details as in Fig. 1. Eth/cont ratio: ratio of DNA microarray values for ethanol treated vs. untreated cells. Act/cont ratio: as in ethanol vs. control treated, but the treatment was with acetaldehyde. C value: the normalized DNA microarray value for each gene's expression in the control specimens. Gene symbols are according to NCBI:Gene.