Table III.
Gene expression analysis of MCF-12A grown in monolayer in the presence or absence of ethanol, or as soft agar selected cell clones compared with control MCF-12A.a
| Monolayer | SA Clone | |||
|---|---|---|---|---|
|
|
|
|||
| Gene ID | Gene description | Eth/cont | Act/cont | SASC/cont |
| Claudin and Integrin families | ||||
| CLDN8 | Claudin 8 | 0.95 | 0.95 | 0.25 |
| CLDN4 | Claudin 4 | 0.9 | 1.0 | 0.21 |
| CLDN7 | Claudin 7 | 0.17 | 0.8 | 0.15 |
| CLDN 1 | Claudin 1 | 0.09 | 0.9 | 0.07 |
| ITGB8 | Integrin, β8 | 1.2 | 1.0 | 3.5 |
| ITGBL1 | Integrin β like 1 | 1.3 | 1.0 | 3.3 |
| ITGA3 | Integrin, α3 | 1.3 | 1.0 | 2.5 |
| ITGB4 | Integrin, β4 | 0.33 | 0.8 | 0.2 |
| ITGB6 | Integrin, β6 | 0.46 | 1.0 | 0.18 |
| ITGA6 | Integrin, α6 | 0.36 | 0.9 | 0.16 |
| Serpin family | ||||
| SERPINE2 | Serpin E2 | 22 | 7.7 | 9 |
| SERPINB1 | Serpin B1 | 1.7 | 1.6 | 3 |
| SERPINH1 | Serpin H1 | 1.5 | 1.2 | 2.9 |
| SERPINB5 | Serpin B5 | 0.6 | 1.0 | 0.3 |
| SERPINA3 | Serpin A3 | 0.5 | 1.0 | 0.2 |
| SERPINB13 | Serpin B13 | 0.1 | 0.7 | 0.06 |
| SERPINB3 | Serpin B3 | 0.05 | 1.0 | 0.02 |
| Keratin and Cadherin families | ||||
| KRT5 | Keratin 5 | 0.6 | 1.0 | 0.32 |
| KRT6C | Keratin 6c | 0.6 | 1.0 | 0.19 |
| KRT16P3 | Keratin 16 pseudogene 3 | 0.3 | 1.1 | 0.1 |
| KRt17 | Keratin 17 | 0.1 | 1.2 | 0.05 |
| KRT16 | Keratin 16 | 0.1 | 1.2 | 0.02 |
| KRT10 | Keratin 10 | 0.1 | 0.3 | 0.01 |
| CDHR1 | Cad rel-member 1 | 22 | 16 | 0.66 |
| CDH2 | Cad 2, t1, N-cadherin | 3 | 1.9 | 4.9 |
| CDH13 | Cad 13, H-cadherin | 1.7 | 1.2 | 0.43 |
| CDH1 | Cad1, type 1, E-cadher | 0.1 | 1.1 | 0.05 |
| CDH3 | Cad 3, t1, P-cadherin | 0.13 | 0.9 | 0.02 |
| Laminin family | ||||
| LAMA4 | α4 | 8.1 | 2.2 | 25 |
| LAMC1 | γ1 | 2.1 | 1.5 | 2.3 |
| LAMA3 | α3 | 0.38 | 0.9 | 0.33 |
| LAMB3 | β3 | 0.32 | 0.7 | 0.07 |
| LAMC2 | γ2 | 0.43 | 1.0 | 0.06 |
a
Gene expression analysis was carried out using Affymetrix human Gene 1.1 ST assays. Eth/cont: ratio of ethanol treated to control (untreated) DNA microarray values. Act/cont: ratio of acetaldehyde to control DNA microarray values. SASC/cont: ratio of soft agar selected clone gene expression to control gene expression. Gene ID: gene identifications according to NCBI:Gene.