Table IV.
Effects of long-term ethanol exposure of MCF-12A cells on the expression of key gene families triggering EMT.a
| SA Clone | Monolayer | |||
|---|---|---|---|---|
|
|
|
|||
| Gene ID | Gene description | SASC/cont | Eth/cont | Act/cont |
| Interleukin family | ||||
| IL7R | Interleukin 7 receptor | 92.1 | 13.5 | 9.1 |
| IL1R1 | Interleukin 1 receptor, type 1 | 10.5 | 8.1 | 5.5 |
| IL6ST | Interleukin 6 signal transducer | 5.1 | 2.2 | 1.4 |
| IL1R2 | Interleukin 1 receptor type II | 3.4 | 10.3 | 10.0 |
| CXCR1 | Chemokine (C-X-C motif) receptor 1 | 0.75 | 1.0 | 1.0 |
| IL22RA1 | Interleukin 22 receptor α1 | 0.44 | 1.0 | 1.0 |
| IL1A | Interleukin 1 | 0.18 | 0.18 | 0.64 |
| IL1RAP | Interleukin 1 receptor accessory protein | 1.29 | 0.59 | 0.83 |
| IL18 | Interleukin 18 (ifn γ inducing factor) | 0.13 | 0.22 | 1.0 |
| IGF family | ||||
| CTGF | Connective tissue growth factor | 11.9 | 10 | 4.6 |
| IGFBP4 | Insulin-like growth factor binding protein 4 | 6.9 | 6.0 | 4.3 |
| IGFBP3 | Insulin-like growth factor binding protein 3 | 5.0 | 4.5 | 2.7 |
| IGFBP2 | Insulin-like growth factor binding protein 2 | 4.0 | 1.7 | 1.0 |
| IGF2R | Insulin-like growth factor 2 receptor | 3.7 | 1.6 | 1.1 |
| IGF2BP3b | Insulin-like GF2 mRNA binding protein 3 | 2.0 | 1.0 | 1.0 |
| Others | ||||
| ANGPT1 | Angiopoieitin | 47 | 15 | 9 |
| FN1b | Fibronectin 1 | 8.7 | 5.8 | 4.5 |
| ZEB2 | Zinc finger E-box binding homeobox 2 | 5.5 | 5.2 | 2.6 |
| EGFR | Epidermal growth factor receptor | 0.49 | 0.44 | 0.76 |
| PPARgC1A | PPARγ coactivator 1α | 3.4 | 1.2 | 1.0 |
| ZEB1 | Zinc finger E-box binding homeobox 1 | 3.3 | 3 | 1.9 |
| TWIST1 | Twist basic helix-loop-helix transcription factor 1 | 2.4 | 6.7 | 4.5 |
| TGFβ and IGF families | ||||
| TGFb2 | Transforming growth factor β2 | 34 | 19 | 7.1 |
| FSL1 | Follistatin-like 1 | 33.6 | 11.5 | 9.3 |
| THBS1 | Thrombospondin 1 | 26.9 | 2.4 | 1.9 |
| CD36 | CD36 molecule (thrombospondin receptor) | 9.5 | 21.1 | 3.9 |
| TGFBR2 | Transforming growth factor, β receptor 2 | 8.6 | 2.4 | 1.3 |
| BMPR1Ab | Bone morphogenetic protein receptor, type 1A | 3.9 | 0.9 | 0.9 |
| BMPR2 | Bone morphogenetic protein receptor, type II | 3.7 | 1.1 | 1.1 |
| TGFBRAP1 | TGFβ associated receptor assoc protein 1 | 3.1 | 0.9 | 0.8 |
| SMAD7 | Smad family member 7 | 0.49 | 1.0 | 1.0 |
| GDF15 | Growth differentiation factor 15 | 0.4 | 0.5 | 0.7 |
| TGFB1 | Transforming growth factor β1 | 0.3 | 1.5 | 1.3 |
| THBD | Thrombomodulin | 0.07 | 0.4 | 0.9 |
| Aldehyde/Alcohol metabolism | ||||
| AOX1 | Aldehyde oxidase 1 | 18.5 | 7.9 | 4.2 |
| ALDH1B1 | Aldehyde dehydrogenase 1 family, member b1 | 4.9 | 1.2 | 1.0 |
| AKR1B1 | Aldo-keto reductase family 1, member B1 | 2.9 | 1.9 | 1.5 |
| ADH5 | Alcohol dehydrogenase 5 | 2.56 | 1.05 | 1.00 |
| ALDH1L2 | Aldhyde dehydrogenase 1 family, member L2 | 2.10 | 0.47 | 0.75 |
| ALDH1A3 | Aldehyde dehydrogenase 1 family, member A3 | 0.43 | 0.75 | 0.86 |
| ALDH3B2 | Aldehyde dehydrogenase 3 family, member B2 | 0.17 | 0.70 | 1.00 |
| AKR1B10 | Aldo-keto reductase family 1, member B10 | 0.01 | 0.14 | 1.46 |
a
Gene expression analysis was carried out using the Affymetrix human Gene 1.1 ST assay.
b
A few genes have multiple probe sets in this assay, so results were averaged. These are indicated after the Gene ID. Gene ID: gene identification according to NCBI:Gene. SASC/cont: ratio of gene expression for soft agar selected cells vs. control cells. Eth/cont and Act/cont refer to ethanol vs. control ratio and acetaldehyde vs. control ratio as in previous tables.