Figure 6. . Gene expression.

(A) Histone methylation enzyme expression in SLE patients. New cohorts of 17 SLE patients and 17 controls were used to examine the expression pattern of some histone methyltransferases and demethylase enzymes. qRT-PCR was used to quantitate the abundance of RNA. JMJD6, CARM1, JMJD3, SET7, SETDB1, EZH2, DOT1L, MML1, MMl2, MML3, MML4, MMl5, SETD1B were examined. Only the four genes shown were significantly different between SLE and control groups. (B) Interferon treatment of THP-1 cells induces changes in the expression of SETD7 and JMJD3. Cells were treated with 1000U/ml IFN-α2 for 18 h. qRT-PCR was used to quantitate the RNA abundance of histone methylation enzymes. In the bar graph, the results are expressed as a fold change from the vehicle-treated sample to normalize across the broad expression levels seen. The error bars represent SEM. Asterisks indicate p < 0.05. n = 3. (C) Interferon treatment of female primary monocytes (as above) changes the expression of several histone methylation enzymes. Asterisks indicate p < 0.05. n = 4. (D) IRF1 was immunoprecipitated from D54MG cells and the blot was probed with anti-JMJD3. Treatments are as indicated.

SLE: Systemic lupus erythematosus.