Figure 7. . Interferon treatment of THP1 cells changes the H3K4me3 at two IRF1 target genes.

Using the peaks identified by ChIP-seq, primers were designed to examine the changes in H3K4me3 at the major promoter peaks seen in systemic lupus erythematosus. There were two IFITM1 peaks at the TSS (A), and upstream (B) regions. There was a single promoter peak at IL18R1 (C). THP1 cells were treated with 1000 U/ml IFN-α2 for 18 h and ChIP assays performed. Interferon treatment induced H3K4me3 at the same peaks seen in the systemic lupus erythematosus patients for these two genes. The error bars represent standard error of the mean. GST is shown as a negative control antibody. The differences at each peak are significant with p < 0.05; n = 4.

GST: Glutathione-S-transferase; TSS: Transcription start site.