Fig 5. Neutrophil reactive oxidant production from subjects with CL before or after treatment.

Neutrophils from CL patients (n = 11) were obtained before and after successful treatment of the disease, in which there was local resolution of the lesion. Cells were incubated in dihydrorhodamine 123 (DHR-123), and incubated with medium alone, 5:1 L. braziliensis, or PMA for ten minutes. The concentration of reactive oxidant species in neutrophils was assessed by flow cytometry. Data represent the median MFI samples from each patient. Statistical analyses were performed using the Wilcoxon test. (**p<0.01, ***p<0.001).