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. Author manuscript; available in PMC: 2016 May 12.
Published in final edited form as: Cell Rep. 2016 Apr 28;15(6):1266–1276. doi: 10.1016/j.celrep.2016.04.010

Figure 6. Contribution of conserved elements in double-domain ENEs to RNA stabilization.

Figure 6

(A) TWIFB1_OSa ENE. Nucleotide shading is as in Figure 2. Mutants designated Mut1-Mut6 were used in the experiments shown in (B). (B) β-globin constructs (βΔ1,2) containing either the WT or a mutant TWIFB1_OSa ENE were transiently expressed in HEK293T cells and analyzed as described in Figure 5. The bars depict the average values from three experiments ±SD.