Figure 5.

Feeding the mice with the docosahexaenoic acid‐enriched diet prior to 48 h fasting prevented adaptations in the endocannabinoid system in the fasted tibialis anterior muscle. Messenger RNA levels for the CB1 receptor, the N‐acyl phosphatidylethanolamine phospholipase D and the fatty acid amide hydrolase were measured by reverse transcription‐quantitative polymerase chain reaction in the tibialis anterior muscle from control and docosahexaenoic acid groups. Data (means ± SEM for n = 5–6 mice/group) are normalized against 18S and expressed as fold induction vs. control fed mice. Statistical differences were assessed by analysis of variance. Bars with different superscript letters are statistically different.