Figure 6.

Feeding the mice with the docosahexaenoic acid‐enriched diet prior to 48 h fasting partially prevented the increased expression of MuRF1 and the accumulation of ubiquitin‐protein conjugates in the fasted tibialis anterior. Messenger RNA levels for MAFbx (A), USP19 (B), and MuRF1 (C) were assessed by reverse transcription‐quantitative polymerase chain reaction in the tibialis anterior from mice fed either the control or the docosahexaenoic acid enriched diet prior to fasting for 48 h. Data are normalized against 18S and are expressed as fold induction vs. control fed mice. (D) High molecular weight ubiquitin–protein conjugates were determined by Western blotting using an antibody that recognize polyubiquitin chains (left panel) in the tibialis anterior from control and docosahexaenoic acid groups. High molecular weight ubiquitin–protein conjugates were quantified (right panel). Data are means ± SEM (n = 5–6 mice/group). Statistical differences were assessed by analysis of variance. Bars with different superscript letters are statistically different.