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. 2016 Feb 15;7(5):587–603. doi: 10.1002/jcsm.12103

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© 2016 The Authors. Journal of Cachexia, Sarcopenia and Muscle published by John Wiley & Sons Ltd on behalf of the Society of Sarcopenia, Cachexia and Wasting Disorders

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Feeding the mice with the docosahexaenoic acid‐enriched diet prior to 48 h fasting resulted in differential adaptations in markers of lipid droplet formation, size, and fate in the fasted tibialis anterior. Messenger RNA levels for PLD1 (A), Rab18 (B), seipin (C), and the perilipins (PLINs)‐2, ‐4, and ‐5 (D) were determined by reverse transcription‐quantitative polymerase chain reaction in the tibialis anterior from control or docosahexaenoic acid groups. Messenger RNA levels were normalized against 18S and expressed as fold induction vs. control fed mice. Data are means ± SEM (n = 5–6 mice/group). Statistical differences were assessed by analysis of variance. Bars with different superscript letters are statistically different.