Table 2.
Primers used for reverse transcription‐quantitative polymerase chain reaction analysis
| Primer names | Accession no | Primer sens sequences | Primer antisens sequences |
|---|---|---|---|
| MAFbx | NM_026346 | 5′‐AGTGAGGACCGGCTACTGTG‐3′ | 5′‐GATCAAACGCTTGCGAATCT‐3′ |
| MuRF1 | NM_001039048 | 5′‐ATGGAGAACCTGGAGAAGCA‐3′ | 5′‐AACGACCTCCAGACATGGAC‐3′ |
| Cthl | NM_009984 | 5′‐CTGTTGCTATGGACGCAAGC‐3′ | 5′‐ACCAACAGAACCCCATGGTC‐3′ |
| Psmb1 | NM_011185 | 5′‐AATTGGCTGCAGTGGTTTCC‐3′ | 5′‐CCGTTGTCATGGCCTTGTTAT‐3′ |
| Psmb2 | NM_011970 | 5′‐AATTGTCTCCCACAGCAGCA‐3′ | 5′‐ATAGCCAGCCAGGAGGAGGT‐3′ |
| Psmb5 | NM_011186 | 5′‐CACCCTGGCCTTCAAGTTTC‐3′ | 5′‐TCACCGTCTGGGAAGCAATA‐3′ |
| Psmd2 | NM_134101 | 5′‐TCTTTGCTATGGGCATGGTG‐3′ | 5′‐GGGTCCTTGGCATGATATTGA‐3′ |
| Psmd3 | NM_009439 | 5′‐CACCCAAGCTGTTAGGACAGG‐3′ | 5′‐TCAGGGTGTAGGTCCCATCC‐3′ |
| Psmd5 | NM_080554 | 5′‐ATTGTTGGCGCAGATTCAGA‐3′ | 5′‐CTATCCATGACGGCCAGGTT‐3′ |
| USP19 | NM_027804 | 5′‐TCCTGTTCGGAACCTGGACT‐3′ | 5′‐TGCCTCCGTAGTGGTTGATG‐3′ |
| Atg5 | NM_053069 | 5′‐TCAACCGGAAACTCATGGAA‐3′ | 5′‐CGGAACAGCTTCTGGATGAA‐3′ |
| Atg12 | NM_026217 | 5′‐TAAACTGGTGGCCTCGGAAC‐3′ | 5′‐CCATCACTGCCAAAACACTCA‐3′ |
| Atg16 | NM_001205391 | 5′‐TCCCGTGATGACCTGCTAAA‐3′ | 5′‐CAGTCAGAGCCGCATTTGAA‐3′ |
| Rab7 | NM_009005 | 5′‐GGGAAACAAGATTGACCTGGA‐3′ | 5′‐CTCCTTGGCACTGGTCTCG‐3′ |
| Pld1 | NM_001164056 | 5′‐ATCGGTGATGGATGGAAAGG‐3′ | 5′‐CCCAGGACAAGTCTGAAGCA‐3′ |
| Bscl2 | NM_001136064 | 5′‐ACCGCTTCTCTCTGCAGGTT‐3′ | 5′‐CCGACTGCTGGGTAGATTCC‐3′ |
| Rab18 | NM_001278447 | 5′‐AGGACGTGCTGACCACTCTG‐3′ | 5′‐TGTGAACCTCAGGAGCAGGC‐3′ |
| Plin2 | NM_007408 | 5′‐GGGTGGAGTGGAAGAGAAGC‐3′ | 5′‐GAGCTGCTGGGTCAGGTTG‐3′ |
| Plin4 | NM_020568 | 5′‐GCTGCATGTGGGAAGCTGT‐3′ | 5′‐GTGCACAGCCTGTCCTGAG‐3′ |
| Plin5 | NM_025874 | 5′‐CCAGTTGGCCACAGTGAATG‐3′ | 5′‐GGCTGATGTCACCACCATGT‐3′ |
| Cnr1 | NM_007726 | 5′‐TCTACGTGGGCTCAAATGACA‐3′ | 5′‐TGGAAGGGACTACCCCTGAA‐3′ |
| 18S | NR_003278 | 5′‐AATCAGTTATGGTTCCTTTGTCG‐3′ | 5′‐GGTCTAGAATTACCACAGTTATCCAA‐3′ |
Atg, autophagy related gene; Bscl2, Berardinelli‐Seip congenital lipodystrophy 2 (seipin); Cnr1, Cannabinoid receptor 1; Cthl, Cathepin L; MAFbx, muscle atrophy F‐box/Atrogin‐1; MuRF1, muscle ring finger 1; plin, perilipin; Pld1, phospholipase D1; Psmb, proteasome subunit beta; Psmd, proteasome 26S subunit, non‐ATPase; Rab7, RAB7 member RAS oncogene family; Rab18, RAB18, member RAS oncogene family; USP, ubiquitin specific peptidase; 18S, 18S ribosomal RNA.