FIG. 7.

Interactions between Ndc80, Mis12 and CENP-T and assembly of the KIN. A. The Spc24/Spc25 heterodimeric RWD domain interacts either with a helical patch on the CENP-T N-terminus (CENP-T helix) or a similar region on the Mis12 subunit Dsn1 (Dsn1 helix). This latter interaction also involves the Mis12 subunit Nsl1 PVIHL motif, which brings the Knl1 tandem RWD domain closer for binding to Spc24/Spc25. Kre28/Zwint homologs have not been identified in filamentous fungi by homology searches. B. Conservation of the CENP-T helix motif from human and ten different fungi (abbreviations as shown in SUPPLEMENTAL TABLE 1). Conserved residues are shown in bold and shaded in grey. C. Conservation of the Dsn1 helix motif from human and ten different fungi (abbreviations as shown in SUPPLEMENTAL TABLE 1) and aligned with the motif shown in B. Conserved residues are shown in bold and shaded in grey and residues that resulted in loss of function in yeast (Malvezzi and others, 2013) are indicated (***). D. Proposed hierarchy of CCAN and KMN assembly in fungi and mammals. See text for details. SAC, spindle assembly checkpoint.