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. Author manuscript; available in PMC: 2017 Jan 25.
Published in final edited form as: Curr Biol. 2016 Jan 7;26(2):263–269. doi: 10.1016/j.cub.2015.11.064

Figure 1. A th2:GFP transgene labels dopaminergic neurons derived from a dlx5/6+ precursor lineage.

Figure 1

(A) Live zebrafish 7 dpf larva expressing th2:GFP with schematic of th2 enhancer/promoter region used for transgenes. (B) Ventral whole-mount view of th2:GFP expression in a dissected 7 dpf brain, box indicates area of posterior recess shown in (C–F). (C) Simultaneous in situ hybridization for th2 mRNA (left) and anti-GFP immunohistochemistry (middle) shows transcript expression in all th2:GFP+ cells (right). Ventral whole-mount view of a dissected 7 dpf brain is shown. (D) Co-expression analysis shows that most th2:GFP+ cells (green) express dlx5/6:mCherry (magenta), indicating their origin from dlx5/6+ precursors. (E) Co-expression analysis shows that most th2:GFP+ cells (green) express dopamine (magenta). (F) Co-expression analysis shows that few th2:GFP+ cells (green) express serotonin (magenta). All images in (D–F) are ventral maximum intensity confocal Z-projections of the hypothalamic posterior recess from dissected 7 dpf brains. Individual and merged channels of boxed regions are shown in lower panels. Scale bar in (B) = 50µm, all other scale bars = 10µM.