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. Author manuscript; available in PMC: 2017 Jan 25.
Published in final edited form as: Curr Biol. 2016 Jan 7;26(2):263–269. doi: 10.1016/j.cub.2015.11.064

Figure 2. th2 + cells are continuously generated throughout life.

Figure 2

(A) 5 dpf larvae were treated with BrdU for 24 hours and analyzed at 9 dpf. Numerous th2:GFP+ cells (green) are labeled with BrdU (magenta) in the hypothalamic posterior recess. Arrowheads indicate double-labeled cells. (B) 12 dpf larvae were treated with BrdU for 24 hours and analyzed at 25 dpf. th2:GFP+ cells (green) labeled with BrdU (magenta) can be found in medial regions of the hypothalamic posterior recess. Arrowheads indicate double-labeled cells. (C) 6 mpf fish were injected interperitoneally with BrdU and analyzed 2 weeks later. th2:GFP+ cells (green) labeled with BrdU (magenta) can be found in a midsagittal view of the hypothalamic posterior recess. Arrowheads indicate double-labeled cells. (D) Percent of BrdU+ cells in the th2:GFP+ population throughout the entire posterior recess. Error bars=SEM, n=5 brains. Images in (A–B) are ventral maximum intensity confocal Z-projections of the hypothalamic posterior recess. Images in (C) are maximum intensity confocal Z-projections from midsagittal views of the hypothalamic posterior recess. Individual and merged channels of boxed region are shown in lower panels. Scale bars=10µM.