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. Author manuscript; available in PMC: 2016 Jul 8.
Published in final edited form as: Mucosal Immunol. 2015 Nov 11;9(4):1039–1050. doi: 10.1038/mi.2015.119

Figure 6.

Figure 6

PCN upregulates sialyl-Lewisx through the PI-PLC pathway. (a) PCN induced the expression of PI-PLC signaling pathway effector PLC-γ2 in NCI-H292 cells exposed to increasing concentrations of PCN. (b-d) NCI-H292 cells were pre-exposed to PI-PLC inhibitor U73122 for 40 minutes before the addition of PBS or PCN (5 μg/ml) for 24 hr. Total cell lysates were used in Western blot analysis using antibodies against (a) PLC-γ2, (b) sialyl-LewisX, (c) C2/4GnT or (d) ST3Gal-IV. For sialyl-LewisX western blot (b), proteins were separated on an agarose-acrylamide gel. β-actin was used for loading controls. Experiments were repeated three times. Representative western blots are shown. Densitometry analyses represent the mean ± standard error of western blots from three independent experiments. *p < 0.05 against PBS control, or in the presence or absence of U73122 by using the one-way ANOVA analysis. #p < 0.05 against PBS control by using the Tukey's test.

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