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. 2015 Nov 19;7(3):377–387. doi: 10.1002/jcsm.12060

Figure 4.

Figure 4

UBE2B knockdown decreased polyUb conjugates levels in fa‐C2C12 myotubes. Immunoblotting was performed on soluble proteins from untreated and Dex‐treated fa‐C2C12 myotubes using antibodies directed against polyUb (all linkages), K63, and K48 conjugates. (A) UBE2B knockdown significantly decreased total polyUb conjugates levels in Dex‐treated myotubes. (B) UBE2B knockdown significantly reduced K48‐linked polyUb conjugates in Dex‐treated myotubes (−28%) but not in untreated cells. (C) PolyUb conjugates via K63 linkages were not significantly modified in either Dex‐treated or untreated fa‐C2C12 myotubes. Load: membranes were stained using Blot‐FastStain dye and densitometric analysis was used to correct for uneven loading (a portion of the membrane is shown). * P < 0.05, n = 6.