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. 2016 May 11;12(5):e1005586. doi: 10.1371/journal.ppat.1005586

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© 2016 Trikin et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 7 — The full-length (myc:full), N-terminally (myc:ΔN) or C-terminally (myc:ΔC) truncated TAC102 was expressed with a triple myc-tag at the N-terminus in PCF cells. Immunofluorescence images show the localization of the tagged proteins. DNA is stained with DAPI (cyan) and myc-tagged proteins (visualized by anti-myc antibody) are shown in magenta. The star (myc:ΔN, day 5) indicates an ancillary kinetoplast and the arrows indicate accumulation of the protein. Scale bar 5 μm. For each of the three cell lines, western blots of digitonin fractionations are shown. ATOM and EF1α are used as fractionation controls. T, total cell lysate; S, supernatant; P, pellet. Fractionations were performed on day 1 post induction. The growth curves show cell growth upon expression of the tagged proteins. The western blots on the right side of the growth curves show expression levels of the tagged versions of TAC102 in comparison to those of the endogenous protein (endo). EF1α is used as a loading control.