Fig. 3.

Suppressive capacity of different myeloid-derived suppressor cell (MDSC) subsets from partiipants with primary glioblastoma. Peripheral blood mononuclear cells (PBMCs) and tumor cell suspensions were stained with CD45, CD11b, CD14, and CD15, and different MDSC subsets were sorted, seeded into anti-CD3–coated plates and co-cultured with CFSE-labeled autologous T-cells at a T-cell-to-MDSC ratio of 2:1 in the presence of IL-2. Five days later, T-cell proliferation was assessed by flow cytometry. (A) Representative dot plots of T-cell proliferation after addition of CD14^highCD15^pos monocytic, CD14^lowCD15^high neutrophilic, and CD14^lowCD15^int MDSCs from the same participant are shown. The percentage values represent the fraction of proliferating CSFE-labeled T-cells. (B) Representative dot plots of T-cell proliferation and intracellular INF-γ secretion after addition of CD14^lowCD15^high neutrophilic MDSCs to CFSE-labeled autologous T-cells at different T-cell -to- MDSC ratios (1:1–4:1) and PMA/ionomycin stimulation. The percentage values represent the fraction of INF-γ secreting CSFE-labeled T-cells. Data are representative of 3 independent experiments done.