Fig. 2.

Marizomib induces apoptosis and caspase-3 activation in glioma cells. (A) FITC Annexin V apoptosis assay kit was used to detect apoptotic cell death induced by marizomib treatment for 24 hours in D-54 cells. (B) DAPI staining was performed to observe apoptotic cells indicated by small, condensed nuclei in D-54 cells treated by marizomib (60 nM) for 24 hours. (C) The caspase-3 activity of D-54 cells was measured using Apopcyto Caspase-3 Fluorometric assay kit 24 hours after treatment with marizomib. An 80% increase in caspase-3 activity was found in presence of 60 nM marizomib. **P < .01, ***P < .001. (D) D-54 and U-251 cells were treated with 60 nM marizomib for indicated time points. Western blot was used to detect cleaved caspase-3 and PARP. Actin was the internal control.