Table 5.
Association of polymorphisms within the genes encoding Toll-like receptors (TLRs) and Triggering receptor expressed on myeloid cells-1 (TREM-1) with polyvascular disease (after the adjustments by age and gender; PVD was defined as intima-media thickness increase ≥ 1 mm or any stenosis of extracranial or/and lower extremity arteries).
| Polymorphism | Inheritance model | Genotype | No PVD | PVD | OR (95% CI) | P value | HWE |
|---|---|---|---|---|---|---|---|
| TLR1 gene | |||||||
| rs5743551 | Codominant | T/T | 31 (79.5%) | 159 (63.4%) | 1.00 | 0.05 | 0.85 |
| C/T | 8 (20.5%) | 83 (33.1%) | 2.09 (0.91–4.78) | ||||
| C/C | 0 (0%) | 9 (3.6%) | NA (0.00–NA) | ||||
| Dominant | T/T | 31 (79.5%) | 159 (63.4%) | 1.00 | 0.035 | ||
| C/T-C/C | 8 (20.5%) | 92 (36.6%) | 2.31 (1.01–5.26) | ||||
| Recessive | T/T-C/T | 39 (100%) | 242 (96.4%) | 1.00 | 0.11 | ||
| C/C | 0 (0%) | 9 (3.6%) | NA (0.00–NA) | ||||
| Overdominant | T/T-C/C | 31 (79.5%) | 168 (66.9%) | 1.00 | 0.089 | ||
| C/T | 8 (20.5%) | 83 (33.1%) | 1.98 (0.87–4.52) | ||||
| Log-additive | – | – | – | 2.32 (1.06–5.07) | 0.022 | ||
| rs5743611 | Codominant | C/C | 22 (56.4%) | 150 (59.5%) | 1.00 | 0.15 | 0.87 |
| C/G | 12 (30.8%) | 91 (36.1%) | 1.07 (0.50–2.27) | ||||
| G/G | 5 (12.8%) | 11 (4.4%) | 0.31 (0.10–0.98) | ||||
| Dominant | C/C | 22 (56.4%) | 150 (59.5%) | 1.00 | 0.63 | ||
| C/G-G/G | 17 (43.6%) | 102 (40.5%) | 0.85 (0.43–1.68) | ||||
| Recessive | C/C-C/G | 34 (87.2%) | 241 (95.6%) | 1.00 | 0.051 | ||
| G/G | 5 (12.8%) | 11 (4.4%) | 0.30 (0.10–0.93) | ||||
| Overdominant | C/C-G/G | 27 (69.2%) | 161 (63.9%) | 1.00 | 0.58 | ||
| C/G | 12 (30.8%) | 91 (36.1%) | 1.23 (0.59–2.56) | ||||
| Log-additive | – | – | – | 0.71 (0.42–1.23) | 0.23 | ||
| TLR2 gene | |||||||
| rs5743708 | – | G/G | 36 (92.3%) | 232 (92.1%) | 1.00 | 0.94 | 0.99 |
| A/G | 3 (7.7%) | 20 (7.9%) | 1.05 (0.30–3.74) | ||||
| rs3804099 | Codominant | T/T | 18 (46.1%) | 105 (41.7%) | 1.00 | 0.84 | 0.17 |
| C/T | 14 (35.9%) | 104 (41.3%) | 1.23 (0.58–2.62) | ||||
| C/C | 7 (17.9%) | 43 (17.1%) | 1.01 (0.39–2.61) | ||||
| Dominant | T/T | 18 (46.1%) | 105 (41.7%) | 1.00 | 0.67 | ||
| C/T-C/C | 21 (53.9%) | 147 (58.3%) | 1.16 (0.59–2.29) | ||||
| Recessive | T/T-C/T | 32 (82%) | 209 (82.9%) | 1.00 | 0.84 | ||
| C/C | 7 (17.9%) | 43 (17.1%) | 0.91 (0.37–2.22) | ||||
| Overdominant | T/T-C/C | 25 (64.1%) | 148 (58.7%) | 1.00 | 0.56 | ||
| C/T | 14 (35.9%) | 104 (41.3%) | 1.23 (0.61–2.49) | ||||
| Log-additive | – | – | – | 1.05 (0.65–1.67) | 0.85 | ||
| TLR4 gene | |||||||
| rs4986790 | Codominant | A/A | 37 (94.9%) | 208 (82.5%) | 1.00 | 0.054 | 0.99 |
| A/G | 2 (5.1%) | 42 (16.7%) | 4.30 (0.99–18.77) | ||||
| G/G | 0 (0%) | 2 (0.8%) | NA (0.00–NA) | ||||
| Dominant | A/A | 37 (94.9%) | 208 (82.5%) | 1.00 | 0.016 | ||
| A/G-G/G | 2 (5.1%) | 44 (17.5%) | 4.42 (1.02–19.20) | ||||
| Recessive | A/A-A/G | 39 (100%) | 250 (99.2%) | 1.00 | 0.55 | ||
| G/G | 0 (0%) | 2 (0.8%) | NA (0.00–NA) | ||||
| Overdominant | A/A-G/G | 37 (94.9%) | 210 (83.3%) | 1.00 | 0.02 | ||
| A/G | 2 (5.1%) | 42 (16.7%) | 4.29 (0.98–18.72) | ||||
| Log-additive | – | – | – | 4.36 (1.01–18.80) | 0.016 | ||
| rs4986791 | Codominant | C/C | 36 (92.3%) | 207 (82.1%) | 1.00 | 0.14 | 0.99 |
| C/T | 3 (7.7%) | 43 (17.1%) | 2.86 (0.83–9.84) | ||||
| T/T | 0 (0%) | 2 (0.8%) | NA (0.00–NA) | ||||
| Dominant | C/C | 36 (92.3%) | 207 (82.1%) | 1.00 | 0.053 | ||
| C/T-T/T | 3 (7.7%) | 45 (17.9%) | 2.94 (0.86–10.07) | ||||
| Recessive | C/C-C/T | 39 (100%) | 250 (99.2%) | 1.00 | 0.55 | ||
| T/T | 0 (0%) | 2 (0.8%) | NA (0.00–NA) | ||||
| Overdominant | C/C-T/T | 36 (92.3%) | 209 (82.9%) | 1.00 | 0.062 | ||
| C/T | 3 (7.7%) | 43 (17.1%) | 2.85 (0.83–9.82) | ||||
| Log-additive | – | – | – | 2.91 (0.86–9.89) | 0.05 | ||
| TLR6 gene | |||||||
| rs3775073 | Codominant | T/T | 18 (46.1%) | 73 (29%) | 1.00 | 0.081 | 0.91 |
| T/C | 13 (33.3%) | 129 (51.2%) | 2.41 (1.11–5.22) | ||||
| C/C | 8 (20.5%) | 50 (19.8%) | 1.57 (0.63–3.91) | ||||
| Dominant | T/T | 18 (46.1%) | 73 (29%) | 1.00 | 0.039 | ||
| T/C-C/C | 21 (53.9%) | 179 (71%) | 2.09 (1.05–4.17) | ||||
| Recessive | T/T-T/C | 31 (79.5%) | 202 (80.2%) | 1.00 | 0.98 | ||
| C/C | 8 (20.5%) | 50 (19.8%) | 0.99 (0.43–2.29) | ||||
| Overdominant | T/T-C/C | 26 (66.7%) | 123 (48.8%) | 1.00 | 0.044 | ||
| T/C | 13 (33.3%) | 129 (51.2%) | 2.05 (1.00–4.18) | ||||
| Log-additive | – | – | – | 1.41 (0.86–2.30) | 0.17 | ||
| rs5743810 | Codominant | G/G | 13 (33.3%) | 110 (43.6%) | 1.00 | 0.042 | 0.52 |
| A/G | 15 (38.5%) | 113 (44.8%) | 0.86 (0.39–1.89) | ||||
| A/A | 11 (28.2%) | 29 (11.5%) | 0.32 (0.13–0.78) | ||||
| Dominant | G/G | 13 (33.3%) | 110 (43.6%) | 1.00 | 0.19 | ||
| A/G-A/A | 26 (66.7%) | 142 (56.4%) | 0.63 (0.31–1.28) | ||||
| Recessive | G/G-A/G | 28 (71.8%) | 223 (88.5%) | 1.00 | 0.013 | ||
| A/A | 11 (28.2%) | 29 (11.5%) | 0.34 (0.15–0.76) | ||||
| Overdominant | G/G-A/A | 24 (61.5%) | 139 (55.2%) | 1.00 | 0.53 | ||
| A/G | 15 (38.5%) | 113 (44.8%) | 1.25 (0.62–2.50) | ||||
| Log-additive | – | – | – | 0.58 (0.36–0.94) | 0.026 | ||
| TREM-1 gene | |||||||
| rs2234246 | Codominant | C/C | 13 (33.3%) | 97 (38.5%) | 1.00 | 0.68 | 0.14 |
| C/T | 20 (51.3%) | 108 (42.9%) | 0.76 (0.36–1.61) | ||||
| T/T | 6 (15.4%) | 47 (18.6%) | 1.08 (0.38–3.04) | ||||
| Dominant | C/C | 13 (33.3%) | 97 (38.5%) | 1.00 | 0.61 | ||
| C/T-T/T | 26 (66.7%) | 155 (61.5%) | 0.83 (0.41–1.71) | ||||
| Recessive | C/C-C/T | 33 (84.6%) | 205 (81.3%) | 1.00 | 0.61 | ||
| T/T | 6 (15.4%) | 47 (18.6%) | 1.27 (0.50–3.21) | ||||
| Overdominant | C/C-T/T | 19 (48.7%) | 144 (57.1%) | 1.00 | 0.38 | ||
| C/T | 20 (51.3%) | 108 (42.9%) | 0.74 (0.37–1.46) | ||||
| Log-additive | – | – | – | 0.98 (0.61–1.58) | 0.95 | ||
| rs4711668 | Codominant | C/C | 15 (38.5%) | 84 (33.5%) | 1.00 | 0.68 | 0.11 |
| T/C | 14 (35.9%) | 104 (41.4%) | 1.42 (0.64–3.14) | ||||
| T/T | 10 (25.6%) | 63 (25.1%) | 1.16 (0.49–2.78) | ||||
| Dominant | C/C | 15 (38.5%) | 84 (33.5%) | 1.00 | 0.45 | ||
| T/C-T/T | 24 (61.5%) | 167 (66.5%) | 1.31 (0.65–2.66) | ||||
| Recessive | C/C-T/C | 29 (74.4%) | 188 (74.9%) | 1.00 | 0.93 | ||
| T/T | 10 (25.6%) | 63 (25.1%) | 0.97 (0.44–2.11) | ||||
| Overdominant | C/C-T/T | 25 (64.1%) | 147 (58.6%) | 1.00 | 0.42 | ||
| T/C | 14 (35.9%) | 104 (41.4%) | 1.33 (0.66–2.71) | ||||
| Log-additive | – | – | – | 1.10 (0.70–1.73) | 0.67 | ||
| rs3804277 | Codominant | C/C | 16 (41%) | 99 (39.3%) | 1.00 | 0.92 | 0.11 |
| C/T | 17 (43.6%) | 108 (42.9%) | 1.05 (0.50–2.20) | ||||
| T/T | 6 (15.4%) | 45 (17.9%) | 1.23 (0.45–3.38) | ||||
| Dominant | C/C | 16 (41%) | 99 (39.3%) | 1.00 | 0.79 | ||
| C/T-T/T | 23 (59%) | 153 (60.7%) | 1.10 (0.55–2.19) | ||||
| Recessive | C/C-C/T | 33 (84.6%) | 207 (82.1%) | 1.00 | 0.7 | ||
| T/T | 6 (15.4%) | 45 (17.9%) | 1.20 (0.47–3.05) | ||||
| Overdominant | C/C-T/T | 22 (56.4%) | 144 (57.1%) | 1.00 | 0.97 | ||
| C/T | 17 (43.6%) | 108 (42.9%) | 0.99 (0.50–1.96) | ||||
| Log-additive | – | – | – | 1.10 (0.68–1.76) | 0.7 | ||
| rs2234237 | Codominant | T/T | 27 (69.2%) | 197 (78.2%) | 1.00 | 0.17 | 0.12 |
| A/T | 9 (23.1%) | 49 (19.4%) | 0.76 (0.34–1.74) | ||||
| A/A | 3 (7.7%) | 6 (2.4%) | 0.22 (0.05–0.98) | ||||
| Dominant | T/T | 27 (69.2%) | 197 (78.2%) | 1.00 | 0.23 | ||
| A/T-A/A | 12 (30.8%) | 55 (21.8%) | 0.63 (0.30–1.32) | ||||
| Recessive | T/T-A/T | 36 (92.3%) | 246 (97.6%) | 1.00 | 0.076 | ||
| A/A | 3 (7.7%) | 6 (2.4%) | 0.24 (0.05–1.02) | ||||
| Overdominant | T/T-A/A | 30 (76.9%) | 203 (80.6%) | 1.00 | 0.65 | ||
| A/T | 9 (23.1%) | 49 (19.4%) | 0.83 (0.37–1.87) | ||||
| Log-additive | – | – | – | 0.59 (0.33–1.08) | 0.1 | ||
| rs6910730 | Codominant | A/A | 28 (71.8%) | 199 (79%) | 1.00 | 0.14 | 0.078 |
| A/G | 8 (20.5%) | 48 (19.1%) | 0.84 (0.36–1.96) | ||||
| G/G | 3 (7.7%) | 5 (2%) | 0.19 (0.04–0.86) | ||||
| Dominant | A/A | 28 (71.8%) | 199 (79%) | 1.00 | 0.3 | ||
| A/G-G/G | 11 (28.2%) | 53 (21%) | 0.66 (0.31–1.42) | ||||
| Recessive | A/A-A/G | 36 (92.3%) | 247 (98%) | 1.00 | 0.052 | ||
| G/G | 3 (7.7%) | 5 (2%) | 0.20 (0.04–0.88) | ||||
| Overdominant | A/A-G/G | 31 (79.5%) | 204 (81%) | 1.00 | 0.83 | ||
| A/G | 8 (20.5%) | 48 (19.1%) | 0.91 (0.39–2.12) | ||||
| Log-additive | – | – | – | 0.60 (0.32–1.11) | 0.11 | ||
| rs1817537 | Codominant | C/C | 16 (41%) | 99 (39.3%) | 1.00 | 0.92 | 0.11 |
| C/G | 17 (43.6%) | 108 (42.9%) | 1.05 (0.50–2.20) | ||||
| G/G | 6 (15.4%) | 45 (17.9%) | 1.23 (0.45–3.38) | ||||
| Dominant | C/C | 16 (41%) | 99 (39.3%) | 1.00 | 0.79 | ||
| C/G-G/G | 23 (59%) | 153 (60.7%) | 1.10 (0.55–2.19) | ||||
| Recessive | C/C-C/G | 33 (84.6%) | 207 (82.1%) | 1.00 | 0.7 | ||
| G/G | 6 (15.4%) | 45 (17.9%) | 1.20 (0.47–3.05) | ||||
| Overdominant | C/C-G/G | 22 (56.4%) | 144 (57.1%) | 1.00 | 0.97 | ||
| C/G | 17 (43.6%) | 108 (42.9%) | 0.99 (0.50–1.96) | ||||
| Log-additive | – | – | – | 1.10 (0.68–1.76) | 0.7 | ||
| rs9471535 | Codominant | T/T | 26 (68.4%) | 195 (77.7%) | 1.00 | 0.15 | 0.13 |
| C/T | 9 (23.7%) | 50 (19.9%) | 0.75 (0.33–1.73) | ||||
| C/C | 3 (7.9%) | 6 (2.4%) | 0.21 (0.05–0.93) | ||||
| Dominant | T/T | 26 (68.4%) | 195 (77.7%) | 1.00 | 0.22 | ||
| C/T-C/C | 12 (31.6%) | 56 (22.3%) | 0.62 (0.29–1.31) | ||||
| Recessive | T/T-C/T | 35 (92.1%) | 245 (97.6%) | 1.00 | 0.068 | ||
| C/C | 3 (7.9%) | 6 (2.4%) | 0.23 (0.05–0.98) | ||||
| Overdominant | T/T-C/C | 29 (76.3%) | 201 (80.1%) | 1.00 | 0.64 | ||
| C/T | 9 (23.7%) | 50 (19.9%) | 0.82 (0.36–1.86) | ||||
| Log-additive | – | – | – | 0.58 (0.32–1.06) | 0.09 | ||
| rs7768162 | Codominant | G/G | 14 (36.8%) | 90 (35.9%) | 1.00 | 0.95 | 0.63 |
| A/G | 18 (47.4%) | 117 (46.6%) | 1.11 (0.52–2.38) | ||||
| A/A | 6 (15.8%) | 44 (17.5%) | 1.14 (0.41–3.18) | ||||
| Dominant | G/G | 14 (36.8%) | 90 (35.9%) | 1.00 | 0.76 | ||
| A/G-A/A | 24 (63.2%) | 161 (64.1%) | 1.12 (0.55–2.29) | ||||
| Recessive | G/G-A/G | 32 (84.2%) | 207 (82.5%) | 1.00 | 0.88 | ||
| A/A | 6 (15.8%) | 44 (17.5%) | 1.07 (0.42–2.74) | ||||
| Overdominant | G/G-A/A | 20 (52.6%) | 134 (53.4%) | 1.00 | 0.86 | ||
| A/G | 18 (47.4%) | 117 (46.6%) | 1.07 (0.53–2.14) | ||||
| Log-additive | – | – | – | 1.08 (0.65–1.77) | 0.77 | ||