Figure EV2. UBE2D1~Ub^Atto discharge assays with TRIM25 and TRIM32 RING mutants.

1. Representative gels of the discharge assays with UBE2D1˜Ub^Atto and different TRIM25 RING mutants. The gels were scanned with a Storm 869 Scanner and the bands for free Ub^Atto integrated.
2. Representative gels for the equivalent assays with TRIM32 RING mutants.