Table 1.
Data collection and refinement statistics
| Crystal | TRIM32 RING | TRIM25/UBE2D1~Ub |
|---|---|---|
| Resolution | 38.56–2.23 (2.31–2.23) | 30.28–2.34 (2.42–2.34) |
| Space group | P43212 | P212121 |
| Cell dimensions | ||
| a,b,c (Å) | 54.54, 54.54, 99.12 | 60.48, 71.70, 160.43 |
| α, β, γ (°) | 90.0, 90.0, 90.0 | 90.0, 90.0, 90.0 |
| R merge | 0.1401 (0.962) | 0.139 (1.918) |
| Total reflections | 161,599 | 384,013 |
| Unique reflections | 7,770 | 30,000 |
| Redundancy | 20.8 | 12.8 |
| Completeness (%) | 99.63 | 99.32 |
| <I/σ(I)> | 13.28 (1.81) | 13.75 (1.53) |
| CC1/2 | 0.996 (0.782) | 0.998 (0.523) |
| Refinement | ||
| R work | 0.248 | 0.227 |
| R free | 0.273 | 0.273 |
| Number of atoms | 1,254 | 4,832 |
| Protein | 1,223 | 4,760 |
| Zn2+ | 4 | 4 |
| Water | 27 | 68 |
| Average B‐factor (Å2) | 85.6 | 56.0 |
| RMS bonds (Å) | 0.002 | 0.002 |
| RMS angles (o) | 0.486 | 0.539 |
| Ramachandran statistics | ||
| Favoured region (%) | 93.2 | 96.11 |
| Allowed region (%) | 6.8 | 3.38 |
| Disallowed region (%) | 0.0 | 0.51 |
Data in the highest resolution shell are shown in parentheses.